GOST 29169-91
(ISO 648-77)

Group P66

INTERSTATE STANDARD

Laboratory glassware

PIPTETTES WITH SINGLE MARK

Laboratory glassware. One-mark pipettes

Date of introduction 1994-01-01

INFORMATION DATA

1. PREPARED AND INTRODUCED by the Klin Independent Design and Technology Bureau for the design of glass instruments and apparatus

2. APPROVED AND ENTERED INTO EFFECT by Resolution of the Committee of Standardization and Metrology of the USSR dated December 16, 1991 N 1952

This standard has been prepared by direct application of the international standard ISO 648-77 "Laboratory glassware. Pipettes with one mark" with additional requirements reflecting the needs of the national economy

3. INSTEAD GOST 20292-74 regarding pipettes with one mark

4. REFERENCE REGULATIVE AND TECHNICAL DOCUMENTS

Number of the section, application in which the link is provided	Designation of the corresponding international standard	Designation of the domestic technical documentation to which the link is given
Annex 1
Annex 1
Section 1, 2, 3, 9; appendices A and B	ISO 384-78

5. REISSUE

1. PURPOSE AND SCOPE OF APPLICATION

This standard covers a range of single mark pipettes for general laboratory use. General requirements- according to GOST 29044.

The requirements of this standard are mandatory.

Additional requirements reflecting the needs of the national economy are given in Appendix 1.

3. BASIC UNITS OF MEASUREMENT

3.1. Capacity unit

The unit of capacity is taken to be a cubic centimeter (cm); the name milliliter (ml) can be used.

Note. The term milliliter (ml) is usually used as a special name for cubic centimeter (cm) according to the International System of Units (SI).

3.2. Standard temperature

Standard temperature, i.e. the temperature at which the nominal volume (nominal capacity) is drained is 20 °C.

Note. When using pipettes in tropical conditions, it is permissible to take the standard water temperature as 27 °C rather than 20 °C, as recommended by GOST 29044.

4. ACCURACY OF VOLUME MEASUREMENT

Two accuracy classes are established:

1st - for higher measurement accuracy;

2nd - for less precise measurements.

5. RANGE OF CAPACITIES

Pipettes with one mark must be manufactured with a nominal capacity of: 0.5; 1; 2; 5; 10; 20; 25; 50; 100 and 200 ml.

For the needs of the national economy, it is allowed to manufacture pipettes with one mark with a nominal capacity of 10.77 ml.

Pipettes with a nominal capacity of 0.5 ml are manufactured without a reservoir, 1 ml - with and without a reservoir, 2 ml - with a reservoir for the 1st class and with and without a reservoir for the 2nd class.

All other sizes are for pipettes with reservoirs.

If necessary, pipettes of all sizes can be manufactured with safety reservoirs above the graduation mark.

Note. If necessary, pipettes of other nominal capacities may be manufactured provided that they comply with the essential requirements of this standard.

6. DETERMINATION OF CAPACITY

The capacity of a single-mark pipette is defined as the volume of water, expressed in milliliters, at 20°C, as described below.

The pipette, in a vertical position, should be filled with liquid a few millimeters above the graduation mark, and the drop of the meniscus should then be adjusted to the mark by one of the methods described below:

A) the meniscus is installed so that the plane of the upper edge of the graduation mark is horizontal tangent to the lower point of the meniscus, the observer’s gaze is in the same plane;

B) the meniscus is installed so that the plane of the center of the mark is horizontal tangent to the lower point of the meniscus. The observer's gaze is directed upward towards this plane, while the front and rear parts of the mark should be simultaneously visible, simultaneously converging at the bottom of the meniscus.

Then remove any drops that adhered to the spout tip when the spout tip came into contact with the surface of the glass container.

After this, the liquid must be drained into another, slightly tilted glass vessel so that the drain tip is in contact with the inner wall of the vessel, but without moving them relative to each other during the drain and waiting period.

The pipette is released until the meniscus drops to the drain hole. To ensure that the drainage has finished, wait 3 s before the pipette is removed from the vessel. This applies to pipettes for which there is no set waiting time.

Note. A waiting period (approximately 3 seconds) is set to determine when to drain the liquid. There is no need to strictly adhere to this time. Be sure that the meniscus has reached the outlet end of the pipette before removing the pipette from the receiving vessel.

If a pipette has a waiting time of 15 s, this time must be maintained before removing the pipette from the receiving vessel.

Note. 1st class pipettes must be:

A) without a specific waiting time, when this time is approximately 3 s;

B) with a waiting time of 15 s.

Class 2 pipettes must have no specified waiting time as defined in subparagraph a.

7. VOLUME PERMISSIBLE ERROR LIMITS

7.1. The limits of permissible error of the nominal capacity should not exceed the values specified in Table 1.

Table 1

Nominal capacity	Permissible error limit
	1st class	2nd class

* For pipettes used for the needs of the national economy.

7.2. The relationship between the nominal capacity and the permissible error limit of the nominal capacity is given in Appendix A, and the relationship between the permissible error limit of the nominal capacity and the meniscus diameter is given in Appendix B.

8. CONSTRUCTION

8.1. Material

Single mark pipettes should be made of glass with adequate chemical and thermal resistance and, if possible, free from visible defects and internal stress.

8.2. Form

Pipettes with a nominal capacity of 0.5 ml consist of a straight tube with a drain hole at the lower end.

Pipettes with a nominal capacity of 1 ml and class 2 pipettes with a nominal capacity of 2 ml may have the design shown in Figure 1 or may have a reservoir as indicated for larger sizes.

All large pipettes consist of a reservoir with a suction and drain tube. These parts must be straight and aligned.

The shape of the pipettes must correspond to that shown in Figure 1.

If a safety reservoir is provided, it is approximately spherical in shape and is located between the graduation mark and the top of the pipette.

For the needs of the national economy, it is allowed to manufacture pipettes with one mark in the following designs:

1 - straight (Fig. 2);

1a - straight lines with a spare tank (Fig. 2);

2 - with extension (Fig. 2);

2a - with expansion and spare tank (Fig. 2).

Example symbol pipettes used for the needs of the national economy, class 1, class 2, nominal capacity 50 ml:

Pipette 1-2-50 GOST 29169-91

8.3. Dimensions

The dimensions of the pipettes must correspond to those indicated in Tables 2 and 3.

table 2

Dimensions in mm

Size name	Nominal capacity, ml

Total length, no more:
straight pipettes
pipettes with reservoir		550; 560****
Length of suction tube*, not less
Length of drain pipe*, not less
Inner diameter at graduation mark**, no more
Outer diameter of drain pipe* (limit deviation ±1 mm)
Tank diameter*, no more
Straight pipette tube diameter, no more

*Only for pipettes with reservoir.

** For straight pipettes - maximum internal diameter; for pipettes with a reservoir - the maximum internal diameter of the suction tube.

*** The estimated minimum distance from the graduation mark to the end of the drain hole is 120 mm (see Table 3).

**** For pipettes used for the needs of the national economy.

Table 3

Size name	Meaning
Distance of the calibration mark from the top of the pipette, not less
Distance from the calibration mark to the top of the tank, not less
Distance from the calibration mark to the drain cone of straight pipettes, not less
Pipette wall thickness, not less:
with a reservoir with a nominal capacity of 1 and 2 ml
other pipettes
Safety tank diameter
Distance from the top of the pipette to the bottom of the safety tank, no more
Distance from the top of the pipette to the top of the safety reservoir, not less

Note. Allowed for pipettes used for the needs of the national economy:

1) the thickness of the expansion wall for pipettes with a reservoir is at least 0.4 mm;

2) the value of the diameter of the safety tank - take it as a size for reference.

The outer diameter of the drain tube and the diameter of the straight pipette tube for pipettes used for national household needs are recommended.

8.4. The top of the pipette should be perpendicular to the axis of the pipette and free from any defects that could interfere with the establishment of the meniscus.

The ends of the glass tubes should be fused or polished smooth with a slight bevel outward.

8.5. Storage tank

The main body of the reservoir is cylindrical, with the exception of pipettes with a nominal capacity of 1 and 2 ml, which may have an oval reservoir if required. The two ends of the tank should gradually transition into the suction and drain pipes. To facilitate drainage of the pipette, the connections should be cone-shaped.

8.6. Drain tip

8.6.1. The drain tip should be strong, smooth, tapered without sharp narrowings at the drain hole.

8.6.2. The end of the drain tip should be treated using one of the methods described below:

A) smoothly ground perpendicular to the axis, slightly beveled outward and melted;

B) smoothly ground perpendicular to the axis and slightly beveled outward;

C) for pipettes used for the needs of the national economy, cut off and melted.

Fire polishing reduces the risk of fracture during use, but should not cause contraction, as specified in paragraph 8.6.1, or internal stress.

8.7. Drain time

The drain time is defined as the time required for the water meniscus to descend from the graduation mark to the drain hole.

The drain time is determined with the pipette in a vertical position and the receiving vessel should be slightly tilted so that the tip of the drain tube is in contact with the inner wall of the vessel, but without moving relative to each other.

Thus, the drain time should be within the limits specified in Table 4.

Table 4

Accuracy class	Drain time, s, for pipettes with nominal capacity, ml

1st class without specific waiting time:


1st class with waiting time 15 s:
no less
no more
2nd class:


Maximum permissible difference between actual and indicated drain time*

* If the draining time is indicated on the pipette in accordance with clause 10.2, then the actual and indicated time values should not differ by more than the value indicated in Table 4.

** For pipettes used for the needs of the national economy.

9. GRADING MARK

The graduation mark must be a clear, uniform, indelible line that completely encircles the tube. It is allowed to break the mark not exceeding 10% of the circumference. The line should be in a plane perpendicular to the axis of the pipette. The line thickness should not exceed 0.4 mm, which is provided for by the requirements of clause 8.2 of GOST 29044.

The dimensions that determine the position of the calibration mark on the pipette are shown in Table 2.

10. LETTERINGS

10.1. All pipettes must be marked with the following markings:

A) a number indicating the nominal capacity and the abbreviation cm or ml to indicate the units in which the pipette is calibrated (see note to clause 3.1);

Note. Where the standard temperature is 27 °C, indicate this value instead of 20 °C;

B) the letter "O", indicating that the pipette is intended to discharge the corresponding nominal capacity;

D) designation 1 or 2 to indicate the accuracy class of graduated pipettes;

D) trademark or name of the manufacturer;

E) the waiting time, if set, in the form "0+15 s".

10.2. Pipettes of the 1st class intended for official verification or certification should be marked with additional markings, which should preferably be applied to other pipettes of the 1st class, and, if necessary, to the pipettes of the 2nd class:

A) designation of this standard;

B) drain time in seconds.

10.3. The maximum permissible error of the nominal capacity in accordance with Table 1 can be applied to all pipettes, for example, ±. . . ml.

11. GRADUATION MARKS, NUMBERS AND INSTRUCTIONS

11.1. All numbers and symbols must be of such sizes and shapes that they are clearly visible when normal conditions applications.

11.2. The graduation mark, numbers and symbols must be clearly visible and indelible.

12. COLOR CODING

If color coding is used, it must be performed in accordance with the requirements of regulatory and technical documentation approved in the prescribed manner.

APPENDIX A

RELATIONSHIP BETWEEN CAPACITY AND PERMISSIBLE ERROR LIMIT FOR CLASS 1 PIPETTES IN ACCORDANCE WITH THE REQUIREMENTS OF clause 4.4 GOST 29044

14.1. Pipettes are packaged in such a way as to ensure their safety during transportation and storage.

14.2. Pipettes packaged according to clause 14.1 are placed in transport containers, where they must be protected from movement and damage.

14.3. Pipettes should be transported in clean covered vehicles.

The text of the document is verified according to:
official publication
Laboratory glassware and equipment.
Pipettes. Burettes: Sat. GOST. -
M.: IPK Standards Publishing House, 2003

GOST 29169-91 (ISO 648-77)

INTERSTATE STANDARD

LABORATORY GLASS TABLEWARE

PIPTETTES WITH SINGLE MARK

Official publication

Standardinform

UDC 542.3:006.354

INTERSTATE

Group P66

STANDARD

Laboratory glassware GOST

PIPTETTES with one MARK

Laboratory glassware. One-mark pipettes

MKS 71.040.20 OKP 43 2133

Date of introduction 01/01/94

1. PURPOSE AND SCOPE OF APPLICATION

This standard covers a range of single mark pipettes for general laboratory use. General requirements - according to GOST 29044.

The requirements of this standard are mandatory.

Additional requirements reflecting the needs of the national economy are given in Appendix 1.

2. LINKS

GOST 29044-91 (ISO 384-78) Laboratory glassware. Principles of arrangement and design of measuring glassware

3. BASIC UNITS OF MEASUREMENT

3.1. Capacity unit

A cubic centimeter (cm 3) is taken as a unit of capacity; the name milliliter (ml) can be used.

Note. The term milliliter (ml) is usually used as a special name for cubic centimeter (cm 3) according to the International System of Units (SI).

3.2. Standard temperature

The standard temperature, i.e. the temperature at which the nominal volume (nominal capacity) is drained, is 20 °C.

Note. When using pipettes in tropical conditions, it is permissible to take the standard water temperature as 27 °C rather than 20 °C, as recommended by GOST 29044.

4. ACCURACY OF VOLUME MEASUREMENT

Two accuracy classes are established:

1st - for higher measurement accuracy;

2nd - for less accurate measurements.

5. RANGE OF CAPACITIES

Pipettes with one mark must be manufactured with a nominal capacity of: 0.5; 1; 2; 5; 10; 20; 25; 50; 100 and 200 ml.

For the needs of the national economy, it is allowed to manufacture pipettes with one mark with a nominal capacity of 10.77 ml.

All other sizes are for pipettes with reservoirs.

Official publication

Reproduction is prohibited

If necessary, pipettes of all sizes can be manufactured with safety reservoirs above the graduation mark.

Note. If necessary, pipettes of other nominal capacities may be manufactured provided that they comply with the essential requirements of this standard.

The capacity of a single-mark pipette is defined as the volume of water, expressed in milliliters, at 20°C, as described below.

a) the meniscus is installed so that the plane of the upper edge of the graduation mark is horizontal tangent to the lower point of the meniscus, the observer’s gaze is in the same plane;

Then remove any drops that adhered to the spout tip when the spout tip came into contact with the surface of the glass container.

If a pipette has a waiting time of 15 s, this time must be maintained before removing the pipette from the receiving vessel.

Note. Class 1 pipettes must be:

a) without a specific waiting time, when this time is approximately 3 s;

b) with a waiting time of 15 s.

Class 2 pipettes must have no specified waiting time as defined in subparagraph a.

6. DETERMINATION OF CAPACITY

Permissible error limit

Nominal capacity

±0.01

±0.02

±0.1

±0.01

±0.02

±0.1

±0.2

8. CONSTRUCTION

8.1. Material

Single mark pipettes should be made of glass with adequate chemical and thermal resistance and, if possible, free from visible defects and internal stress.

Pipettes with a nominal capacity of 0.5 ml consist of a straight tube with a drain hole at the lower end.

Pipettes with a nominal capacity of 1 ml and class 2 pipettes with a nominal capacity of 2 ml may have the following drawing. 1 design or may have a reservoir as indicated for larger sizes.

All large pipettes consist of a reservoir with a suction and drain tube. These parts must be straight and aligned.

The shape of the pipettes must correspond to that shown in Fig. 1.

If a safety reservoir is provided, it is approximately spherical in shape and is located between the graduation mark and the top of the pipette.

For the needs of the national economy, it is allowed to manufacture pipettes with one mark in the following designs:

1 - straight (Fig. 2);

1a - straight lines with a spare tank (Fig. 2);

2 - with extension (Fig. 2);

2a - with expansion and spare tank (Fig. 2).

Execution 1 Execution 1a Execution 2 Execution 2a

An example of a symbol for a pipette used for the needs of the national economy, class 1, class 2, nominal capacity 50 ml:

Pipette 1-2-50 GOST 29169-91

8.3. Dimensions

The dimensions of the pipettes must correspond to those indicated in the table. 2 and 3.

Dimensions in mm

table 2

Size name	Nominal capacity, ml
Size name
Total length, no more: straight pipettes pipettes with reservoir
Length of suction tube 1), not less
Length of drain pipe 1), not less
Inner diameter at graduation mark 2), no more
Outer diameter of drain pipe 1) (limit deviation ±1 mm)
Tank diameter 1), no more
Straight pipette tube diameter, no more

^Only for pipettes with reservoir.

^ For straight pipettes - maximum internal diameter; for pipettes with a reservoir - the maximum internal diameter of the suction tube.

^ The calculated minimum distance from the graduation mark to the end of the drain hole is 120 mm (see Table 3).

^ For pipettes used for the needs of the national economy.

The outer diameter of the drain tube and the diameter of the straight pipette tube for pipettes used for national household needs are recommended.

Table 3

Size name

Meaning

Distance of the graduation mark from the top of the pipette, not less Distance from the graduation mark to the top of the reservoir, not less Distance from the graduation mark to the drain cone of straight pipettes, not less

Pipette wall thickness, not less: with a reservoir with a nominal capacity of 1 and 2 ml of other pipettes

Safety tank diameter

Distance from the top of the pipette to the bottom of the safety reservoir, no more Distance from the top of the pipette to the top of the safety reservoir, no less

Note. Allowed for pipettes used for the needs of the national economy:

1) the thickness of the expansion wall for pipettes with a reservoir is at least 0.4 mm;

2) the value of the diameter of the safety tank - take it as a size for reference.

8.4. The top of the pipette should be perpendicular to the axis of the pipette and free from any defects that could interfere with the establishment of the meniscus.

The ends of the glass tubes should be fused or polished smooth with a slight bevel outward.

8.5. Storage tank

8.6. Drain tip

8.6.1. The drain tip should be strong, smooth, tapered without sharp narrowings at the drain hole.

8.6.2. The end of the drain tip should be treated using one of the methods described below:

a) smoothly ground perpendicular to the axis, slightly beveled outward and melted;

b) smoothly ground perpendicular to the axis and slightly beveled outward;

c) for pipettes used for the needs of the national economy, cut off and melted.

Fire polishing reduces the risk of fracture during use, but should not cause narrowing, as indicated in and. 8.6.1, or internal stress.

8.7. Drain time

The drain time is defined as the time required for the water meniscus to descend from the graduation mark to the drain hole.

Thus, the drain time must be within the limits specified in the table. 4.

Table 4

Accuracy class		Drain time, s, for pipettes with nominal capacity,
Accuracy class
1st class without a specific waiting time: no less

1st class with waiting time 15 s: no less

2nd class: no less

The maximum permissible difference between the actual and indicated drain time

^ If the drainage time is indicated on the pipette in accordance with and. 10.2, then the actual and designated time values should not differ by more than the value indicated in the table. 4.

^ For pipettes used for the needs of the national economy.

9. GRADING MARK

The graduation mark must be a clear, uniform, indelible line that completely encircles the tube. A break in the mark not exceeding 10% of the circumference is allowed. The line should be in a plane perpendicular to the axis of the pipette. The thickness of the line should not exceed 0.4 mm, which is provided for by the requirements and. 8.2 GOST 29044.

The dimensions that determine the position of the calibration mark on the pipette are shown in table. 2.

10. LETTERINGS

10.1. All pipettes must be marked with the following markings:

a) a number indicating the nominal capacity and the abbreviation cm 3 or ml to indicate the units in which the pipette is calibrated (see note to clause 3.1);

Note. Where the standard temperature is 27 °C, indicate this value instead of 20 °C;

c) the letter “O” indicating that the pipette is designed to discharge the corresponding nominal capacity;

d) designation 1 or 2 to indicate the accuracy class of graduated pipettes;

e) trademark or name of the manufacturer;

f) the waiting time, if set, in the form “0+15 s”.

a) designation of this standard;

b) drain time in seconds.

10.3. Limit of permissible error of nominal capacity in accordance with table. 1 can be applied to all pipettes, for example ±... ml.

11. GRADUATION MARKS, NUMBERS AND INSTRUCTIONS

11.1. All numbers and symbols must be of such size and shape as to be clearly visible under normal conditions of use.

11.2. The graduation mark, numbers and symbols must be clearly visible and indelible.

12. COLOR CODING

If color coding is used, it must be performed in accordance with the requirements of regulatory and technical documentation approved in the prescribed manner.

APPENDIX A

RELATIONSHIP BETWEEN CAPACITY AND PERMISSIBLE ERROR LIMIT FOR CLASS 1 PIPETTES IN ACCORDANCE WITH THE REQUIREMENTS OF clause 4.4 GOST 29044

APPENDIX B

RELATIONSHIP BETWEEN THE LIMITS OF PERMISSIBLE ERROR AND THE DIAMETER OF THE MENISCUS FOR PIPETTES 1ST CLASS IN ACCORDANCE WITH THE REQUIREMENTS OF clause 4.7 GOST 29044

Limit of permissible error for Hgo class, ± µl

Meniscus diameter, mm

ANNEX 1

ADDITIONAL REQUIREMENTS REFLECTING THE NEEDS OF THE NATIONAL ECONOMY

13. Test methods

13.1. The nominal capacity of pipettes should be checked in accordance with the regulatory and technical documentation approved in the prescribed manner.

13.2. The dimensions of pipettes (i. 8.3) should be checked with a universal measuring instrument in accordance with GOST 166 and GOST 427.

13.3. The drainage time of the pipettes should be checked by and. 8.7 of this standard.

14. Packaging, transportation and storage

14.1. Pipettes are packaged in such a way as to ensure their safety during transportation and storage.

14.2. Pipettes packaged by and. 14.1, are placed in transport containers, where they must be protected from movement and damage.

14.3. Pipettes should be transported in clean covered vehicles.

INFORMATION DATA

1. PREPARED AND INTRODUCED by the Elinsky Independent Design and Technology Bureau for the design of glass instruments and apparatus

2. APPROVED AND ENTERED INTO EFFECT by Resolution of the Committee of Standardization and Metrology of the USSR dated December 16, 1991 No. 1952

This standard has been prepared by direct application of the international standard ISO 648-77 “Laboratory glassware. Pipettes with one mark" with additional requirements reflecting the needs of the national economy

3. INSTEAD GOST 20292-74 regarding pipettes with one mark

4. REFERENCE REGULATIVE AND TECHNICAL DOCUMENTS

5. REPUBLICATION. March 2008

Approved Decree of the USSR Committee of Standardization and Metrology of December 16, 1991 N 1952

State standard USSR GOST 29169-91 (ISO 648-77)

"LABORATORY GLASS CONSTRUCTION. PIPTES WITH SINGLE MARK"

Laboratory glassware. One-mark pipettes

1. Purpose and scope

This standard covers a range of single mark booties for general laboratory use. General requirements - according to GOST 29044 (ISO 384).

The requirements of this standard are mandatory.

Additional requirements reflecting the needs of the national economy are given in Appendix 1.

2. Links

GOST 29044 (ISO 384) "Laboratory glassware. Principles of arrangement and design of measuring glassware."

3. Basic units of measurement

3.1. Capacity unit

A cubic centimeter (cm 3) is taken as a unit of capacity; the name milliliter (ml) can be used.

Note. Ternia milliliter (ml) is usually used as a special name for cubic centimeter (cm 3) according to the International System of Units (SI).

3.2. Standard temperature

Standard temperature, i.e. the temperature at which the nominal volume (nominal capacity) is drained is 20°C.

Note. When using pipettes in tropical conditions, it is permissible to take the standard water temperature as 27°C rather than 20°C, as recommended by GOST 29044 (ISO 384).

4. Volume measurement accuracy

Two accuracy classes are established:

1st - for higher measurement accuracy;

2nd - for less precise measurements.

5. Range of capacities

Single mark pipettes should be manufactured to a nominal capacity; 0.5; 1; 2; 5; 10; 20; 25; 50; 100 and 200 ml.

For the needs of the national economy, it is allowed to manufacture pipettes with one mark with a nominal capacity of 10.77 ml.

All other sizes are for pipettes with reservoirs.

If necessary, pipettes of all sizes can be manufactured with safety reservoirs above the graduation mark.

Note. If necessary, pipettes of other nominal capacities may be manufactured provided that they comply with the essential requirements of this standard.

6. Determination of capacity

The capacity of a single-mark pipette is defined as the volume of water, expressed in milliliters, at 20°C, as described below.

a) the meniscus is installed so that the plane of the upper edge of the graduation mark is horizontal tangent to the lower point of the meniscus, the observer’s gaze is in the same plane;

Then remove any drops that adhered to the spout tip when the spout tip came into contact with the surface of the glass container.

If a pipette has a waiting time of 15 s, this time must be maintained before removing the pipette from the receiving vessel.

Note. 1st class pipettes must be:

a) without a specific waiting time, when this time is approximately 3 s;

b) with a waiting time of 15 s.

Class 2 pipettes must have no specified waiting time as defined in subparagraph a.

7. Limits of permissible error by volume

7.1. The limits of permissible error of the nominal capacity should not exceed the values specified in table. 1.

Table 1

Nominal capacity	Permissible error limit
Nominal capacity

_____________________________

* For pipettes used for national economic needs

7.2. The relationship between the nominal capacity and the maximum permissible error of the nominal capacity is given in Appendix A, and the relationship between the maximum permissible error of the nominal capacity and the diameter of the meniscus is given in Appendix B.

8. Design

8.1. Material

Single mark pipettes should be made of glass with adequate chemical and thermal resistance and, if possible, free from visible defects and internal stress.

Pipettes with a nominal capacity of 0.6 ml consist of a straight tube with a drain hole at the lower end.

Pipettes with a nominal capacity of 1 ml and class 2 pipettes with a nominal capacity of 2 ml may have the following drawing. 1 design or may have a reservoir as indicated for larger sizes.

All large pipettes consist of a reservoir with a suction and drain tube. These parts must be straight and aligned.

The shape of the pipettes must correspond to that shown in Fig. 1.

"Diagram 1. Pipette shape"

If a safety reservoir is provided, it is approximately spherical in shape and is located between the graduation mark and the top of the pipette.

For the needs of the national economy, it is allowed to manufacture pipettes with one mark in the following designs:

1 - straight (Fig. 2);

"Diagram 2. Options for pipettes with one mark"

1a - straight lines with a spare tank (Fig. 2);

2 - with extension (Fig. 2);

2a - with expansion and spare tank (Fig. 2).

An example of a symbol for a pipette used for the needs of the national economy, class 1, class 2, nominal capacity 50 ml:

Pipette 1 - 2 - 50 GOST 29169-91

8.3. Dimensions

The dimensions of the pipettes must correspond to those indicated in the table. 2 and 3.

table 2

Dimensions in mm

Size name	Nominal capacity, ml
Size name
Total length, no more:
straight pipettes
pipettes with reservoir
Length of suction tube*(1), not less
Drain pipe length*(1), not less
Inner diameter at graduated mark*(2), no more
Outer diameter of drain pipe*(1) (limit deviation ±1 mm)
Tank diameter*(1), no more
Straight pipette tube diameter, no more

_____________________________

*(1) For reservoir pipettes only.

*(2) For straight pipettes - maximum internal diameter; for pipettes with a reservoir - the maximum internal diameter of the suction tube.

*(3) The estimated minimum distance from the graduation mark to the end of the drain hole is 120 mm (see Table 3).

*(4) For pipettes used for the needs of the national economy.

The outer diameter of the drain tube and the diameter of the straight pipette tube for pipettes used for national household needs are recommended.

Table 3

Size name	Meaning
Distance of the calibration mark from the top of the pipette, not less
Distance from the calibration mark to the top of the tank, not less
Distance from the calibration mark to the drain cone of straight pipettes, not less
Pipette wall thickness, not less:
with a reservoir with a nominal capacity of 1 and 2 ml
other pipettes
Safety tank diameter
Distance from the top of the pipette to the bottom of the safety tank, no more
Distance from the top of the pipette to the top of the safety reservoir, not less

Note. Allowed for pipettes used for the needs of the national economy:

1) the thickness of the expansion wall for pipettes with a reservoir is at least 0.4 mm;

2) the value of the diameter of the safety tank - take it as a size for reference.

8.4. The top of the pipette should be perpendicular to the axis of the pipette and free from any defects that could interfere with the establishment of the meniscus.

The ends of the glass tubes should be fused or polished smooth with a slight bevel outward.

8.5. Storage tank

8.6. Drain tip

8.6.1. The drain tip should be strong, smooth, tapered without sharp narrowings at the drain hole.

8.6.1. The end of the drain tip should be treated using one of the methods described below:

a) smoothly ground perpendicular to the axis, slightly beveled outward and melted;

b) smoothly ground perpendicular to the axis and slightly beveled outward;

c) for pipettes used for the needs of the national economy, cut off and melted.

Fire polishing reduces the risk of fracture during use, but should not cause contraction, as specified in paragraph 8.6.1, or internal stress.

8.7. Drain time

The drain time is defined as the time required for the water meniscus to descend from the graduation mark to the drain hole.

Thus, the drain time must be within the limits specified in the table. 4.

Table 4

Drain time, s

Accuracy class	For pipettes with nominal capacity, ml
Accuracy class
1st class without specific waiting time:


1st class with waiting time 15 s:


2nd grade;


Maximum permissible difference between actual and indicated drain time*(1)

_____________________________

*(1) If the draining time is indicated on the pipette in accordance with clause 10.2, then the actual and indicated time values should not differ by more than the value indicated in the table. 4,

*(2) For pipettes used for the needs of the national economy.

9. Graduation mark

The graduation mark must be a clear, uniform, indelible line that completely encircles the tube. It is allowed to break the mark not exceeding 10% of the circumference. The line should be in a plane perpendicular to the axis of the pipette. The thickness of the line should not exceed 0.4 mm, which is provided for by the requirements of clause 8.2 of GOST 29044 (ISO 384).

The dimensions that determine the position of the calibration mark on the pipette are shown in table. 2.

10. Inscriptions

10.1. All pipettes must be marked with the following markings:

a) a number indicating the nominal capacity and the abbreviation cm 3 or ml to indicate the units in which the pipette is calibrated (see note to clause 3.1);

Note. So, where the standard temperature is 27°C, this value is called instead of 20°C;

c) the letter "O", indicating that the pipette is intended to discharge the corresponding nominal capacity;

d) designation 1 or 2 to indicate the accuracy class of graduated pipettes;

e) trademark or name of the manufacturer;

f) the waiting time, if set, in the form "0 + 15 s".

10.2. Class 1 pipettes intended for official verification or certification should be marked with additional markings, which should preferably be applied to other class 1 pipettes and, if necessary, to class 2 pipettes;

a) designation of this standard;

b) drain time in seconds.

10.3. Limit of permissible error of nominal capacity in accordance with table. 1 can be applied to all pipettes, for example±...ml.

11. Graduation marks, numbers and inscriptions

11.1. All numbers and symbols must be of such size and shape as to be clearly visible under normal conditions of use.

11.2. The graduation mark, numbers and symbols must be clearly visible and indelible.

12. Color coding

If color coding is used, it must be performed in accordance with the requirements of regulatory and technical documentation approved in the prescribed manner.

Appendix A

The relationship between capacity and permissible error limit for class 1 pipettes in accordance with the requirements of clause 4.4 of GOST 29044 (ISO 384)

Appendix B

The relationship between the limits of permissible error and the diameter of the meniscus for class 1 pipettes in accordance with the requirements of clause 4.7 of GOST 29044 (ISO 384)

Annex 1

Additional requirements reflecting the needs of the national economy

13. Test methods

13.1. The nominal capacity of pipettes should be checked in accordance with the regulatory and technical documentation approved in the prescribed manner.

13.2. The dimensions of the pipettes (clause 8.3) should be checked with a universal measuring instrument in accordance with GOST 166 and GOST 427.

13.3. The draining time of pipettes should be checked according to clause 8.7 of this standard.

14. Packaging, transportation and storage

14.1. Pipettes are packaged in such a way as to ensure their safety during transportation and storage.

14.2. Pipettes packaged according to clause 14.1 are placed in transport containers, where they must be protected from movement and damage.

14.3. Pipettes should be transported in clean covered vehicles.

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GOST 29169-91 (ISO 648-77)

PIPTETTES WITH SINGLE MARK

Official publication

INTERSTATE STANDARD

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Laboratory glassware GOST

LABORATORY GLASS TABLEWARE

UDC 542.3:006.354 Group P66

29169-91 PIPTES WITH SINGLE MARK

(ISO 648-77)

GaBboga zama One-mark arena

1. PURPOSE AND SCOPE OF APPLICATION

ISS TEN. 20 OKP 3+3 2115

Daga introduction 01/01/94

This standard applies to a number of fully marked pipettes for general laboratory use. General requirements according to GOST 29044.

The requirements of this standard are mandatory.

3. BASIC UNITS OF MEASUREMENT

Additional requirements. reflecting the needs of the national economy are given in Appendix 1.

GOST 29044 EEZISO 334 78) Laboratory glass container. Principles of arranging and constructing a measuring promise

3.1. Unit of capacity A unit of capacity is taken to be a cubic centimeter (cm”). It is forbidden to use the name milliliter (ml).

Note. The term mialiliter (usually used as a special name for a centimeter in accordance with the International System of Units SIT

4. ACCURACY OF VOLUME MEASUREMENT

Set two accuracy classes: I - for higher measurement accuracy: 2nd for less accurate measurements.

5. RANGE OF CAPACITIES

Pipettes with one mark must be manufactured with a nominal capacity of: 0.5. [; 2:9:K:20; 25; M: Not 200 ml.

To meet the needs of the national economy, it is also possible to supply pipettes with one mark with a nominal capacity of 10.77 ml.

Pipettes with a nominal capacity of 0.5 mA are manufactured without a reservoir. [ ml with and without reservoir. 2 ml with tank for 1st class bark and with and without tank for 2nd class.

All other sizes are for pipettes with reservoirs.

Official publication Reprint reproduced

\Standart Publishing House. 1992 © Stanlartinfurm. 208

S. 2 GOST 29169-91

If necessary, pipettes of all sizes can be supplied with preservative reservoirs with a sealing mark.

Note It is necessary to use pipettes of other nominal capacities when using potions. They will correspond to the main requirements of this stanlar.

6. DETERMINATION OF CAPACITY

The tablet in a vertical position should be filled with liquid a few millimeters above the grading mark. and the meniscus flare should then be adjusted to the mark with one of the tools. described below:

a) the meniscus is installed like this. so that the plane of the upper edge of the finishing mark is horizontal and tangent to the lower point of the meniscus. The observer's wobble will be in the same plane:

6) the meniscus is installed like this. so that the plane of the nengra mark is horizontal tangent to the lower point of the meniscus. The observer's eye is directed upward towards this plane. When scrapping, the pouring and filling parts of the mark must be formed at the same time. simultaneously fused in the lower parts of the meniscus.

Then all the drops are removed. close to the tip at the moment of contact of the drain pipe with the surface of the glass vessel.

After this, the vein can be easily drained into another. a slightly bowed glass vessel like this. so that the drain tip is in contact with the inner wall of the vessel. but without moving them relative to each other during the drain and the waiting period.

The inlet has been released since then. until the drain hole drops down. Just to be sure. why the drain has ended. Zs come to life. before the pipette is removed from the vessel. This applies to pipettes. For whom there is no waiting time.

Note. The vitality indicator approximately No. c) is included in determining the time for draining the liquid. There is no need to strictly adhere to this time. You need to be sure. chi msnisk reached the glorious edge of DUCT RI, before taking out the post of Igoriem's sucker.

If the pipette barks, the revival time is set to 15 sec. Now is the time to be respected as before. than to remove the pill from the receiving sucker.

Note. 1st class pilettes must be

i) all: a certain time of life. when this time is approximately equal to Ze.

$) with a revival time of 15 s.

2nd class pins must be subject to a specific waiting time as defined by i.a.

7. VOLUME PERMISSIBLE ERROR LIMITS

7.1. The bursting capacity limits of the rated capacity should not exceed the value. indicated in table. 1.

Table 1

Prezel zopuskaechon error

Nominal capacity

2 and grade

5 10.43 10 21.04 1.77 #0413 m:0.06 28 20.06 89 #0. 1

14) 21.1% 24H) 2.2

on Aa RE LOK, barking OTP COMOCTOH HUG VOUT Bab

GOST 29169-91 S. 3

7.2. The relationship between the nominal capacity and the margin of error of the nominal capacity is given in Appendix A. and the ratio of the margin of error of the nominal capacity and the diameter of the meniscus in Appendix B.

8. CONSTRUCTION

$.1. Material

Pyrettes with a full mark must be made of glass with appropriate chemical and thermal resistance. if possible. free from visible defects and internal tension.

Pipettes with a nominal capacity of 0.5 ml consist of a straight tube with a drain hole at the lower end.

Booties rated capacity | ml and class 2 pipettes with a nominal capacity of 2 ml can have the following characteristics. | structure or may have a reservoir. as indicated for larger sizes.

All large pipettes consist of a reservoir with a suction tube and a drain tube. These parts must be straight and aligned.

The shape of the pipettes must correspond to that shown in the drawing. 1.

If a safety reservoir is provided. it is approximately spherical in shape and is located between the graduation mark and the top of the bootie.

It is allowed for the needs of the national economy and the heating of booties with one mark of the following design:

| straight (Fig. 2);

1a straight lines with a spare tank (Fig. 2);

2 with extension (Fig. 29

2 with expansion and reserve tank (Fig. 2).

Sweating (Execution ba Execution 2 No.

S. $ GOST 29169-91

nN is an example of the conventional designation of a pipette. used according to the needs of the national economy. execution |. 2nd class. nominal capacity 50 ml:

Pipette 2 ZOTOST 29 9 8.3. Dimensions The dimensions of the pills must correspond to those indicated in the table. 2 and 3.

Taolinia 2

Dimensions nmm

Nominal capacity. m1

Olina iia, He Gade. spicy lined booties with a reserve

OSU Length of the side of the drain pipe", not less than 160 (Tu Length of the drain tube", not less than 160 240 4 water tank He foley 4.8 &.5 Outer lysmegr of the drain tube "approx. mm! 6.8 9 Tank diameter! no more than 16 449

He Gotee Diameter

11. Only 44th tank

` Danya pipette - maximum internal diameter. For those with a reservoir - the maximum internal diameter of the non-collapsing tube.

“The estimated minimum distance from the trawling mark to the end of the drain hole is 1209 mm. those 3).

4) Dalia booties. exhausted ala potreonosti and narolnogo

Table 3

Name lazmeya Meaning

The distance of the calibration mark og the top part of the pipette, not less than Distance og:

marks 1 of the top of the tank, no chance

10 Distance og of the trawl line mark ao of the drain cone for collecting straight pipettes. no less than fu Goinina walls of the tsineti, no less than: © reservoir with nominal capacity Gi 2 ma 0.7 drurnkh MLN TOR tu Diameter of the protective reservoir 25 Distance of the upper part of the pistka lezna of the protective reservoir. no more than Tu Distance from the upper part of the pinest to the upper part of the protective tank. m no less

Note. It is allowed to bark pinetak, bark potreonosti folk 1! The thickness of the expansion wall ala piestek with tanks is at least 0.4 mm.

21 value of the diameter of the protective reservoir - taken as the size of the certificate.

GOST 29169-91 S. 5

$.4. The upper part of the bootie must be perpendicular to the axis of the bootie and free from any defects. which may interfere with the establishment of the meniscus.

The ends of the glass tubes should be melted or smoothly polished with a slight bevel outward.

$.5. Storage tank

The main part of the reservoir is nilinaric. with the exception of pipettes with a nominal capacity of 2 ml. which may have. if necessary. oval tank. Two containers of the tank should be gradually poured into the suction and drain pipes. To facilitate drainage of the pipette, the connections should be cone-shaped.

8.6. Drain tip

5.6.1. The drain tip must be strong. Glalkim. conical without sharp narrowing at the drain hole.

$.6.2. The ends of the drain tip should be treated with one of the methods. described below:

a) smoothly ground and perpendicular to the axis. slightly beveled outwards and melted:

6) smoothly polished perpendicular to the axis and slightly beveled outward:

c) for pipettes. used for the needs of the national economy. cut off and melted.

Fire polishing reduces the risk of damage during use. but should not cause contraction. as specified in clause 8.6.1. or morning tension.

8.7. Drain time

The drain time is defined as the time required for the water meniscus to lower from the grading mark to the drain hole.

The drainage time is determined with the pipette in a vertical position, and the receiving vessel should be slightly tilted so. so that the tip of the drain tube is in contact with the inner film of the sucker. but without movement relative to each other's springs.

Gakim obrayum. It’s time to drain and it’s wrong to soak in the delights. indicated in table. 4.

Taolina 4

Accuracy class

1-Fet is destined!

Denmark 15 s: no less > He boss = 2-H He 40) no more than 29 Maximum zonsky

risity of leisawitsl-

time and time

it's a drain!" 2 2 2 3 3 4 4 4 5 5 3

te Esai the time of sain is indicated on pipstya in p. 19.2. 1" The real and designated time values should not differ by more than the value indicated in Table 4 of Dali. using the needs of the people

9. GRADING MARK

The graduation mark must be clear. uniform. indelible line. completely surrounding the tube. A break in the mark not exceeding 10" of the circumference is allowed. The line should lie flat. perpendicular to the axis of the pipette. The line thickness should not exceed 0.3 mm. which is provided for by the requirements of paragraph $.2 of GOST 29044.

Dimensions. determining the position of the grading mark on the pipette. are indicated in table. 2.

S. 6 GOST 29169-91

10. LETTERINGS

10.1. All pirettes must bear the following markings:

a) number. indicating the nominal capacity and abbreviation cm? or ml ala unit designations. in which the pipette is calibrated (see note to clause 3.1):

6) 20 °C (standard recommended temperature).

Note. There. When the average temperature is 27 °C, this value is indicated instead of 20 °C.

c) the letter “Oe”, indicating that. that the pipette is intended to discharge the corresponding nominal capacity:

d) designation | nai 2 to indicate the accuracy class of granulated pirettes.

i) trademark or name of the enterprise

10.2. On the class pipettes. intended for official verification or certification. Additional markings must be applied. which is preferably applied to other pills of class II. and when necessary. for class 2 pipettes:

a) designation of this standard:

6) drain time in seconds.

10.3. Limit of permissible error of nominal capacity in accordance with table. 1 can be applied to all pipettes. for example +... ml.

Il. GRADUATION MARKS, NUMBERS AND INSTRUCTIONS

GOST 29169-91
(ISO 648-77)

Group P66

INTERSTATE STANDARD

Laboratory glassware

PIPTETTES WITH SINGLE MARK

Laboratory glassware. One-mark pipettes

ISS 71.040.20
OKP 43 2133

Date of introduction 1994-01-01

INFORMATION DATA

1. PREPARED AND INTRODUCED by the Klin Independent Design and Technology Bureau for the design of glass instruments and apparatus

2. APPROVED AND ENTERED INTO EFFECT by Resolution of the Committee of Standardization and Metrology of the USSR dated December 16, 1991 N 1952

This standard has been prepared by direct application of the international standard ISO 648-77 * "Laboratory glassware. Pipettes with one mark" with additional requirements reflecting the needs of the national economy
________________
* Access to international and foreign documents mentioned here and further in the text can be obtained by following the link to the website http://shop.cntd.ru. - Database manufacturer's note.

3. INSTEAD GOST 20292-74 regarding pipettes with one mark

4. REFERENCE REGULATIVE AND TECHNICAL DOCUMENTS


Number of the section, application in which the link is provided	Designation of the corresponding international standard	Designation of the domestic technical documentation to which the link is given
Annex 1
Annex 1
Section 1, 2, 3, 9; appendices A and B	ISO 384-78

5. REPUBLICATION. March 2008

1. PURPOSE AND SCOPE OF APPLICATION

This standard covers a range of single mark pipettes for general laboratory use. General requirements - according to GOST 29044.

The requirements of this standard are mandatory.

Additional requirements reflecting the needs of the national economy are given in Appendix 1.

2. LINKS

GOST 29044-91 (ISO 384-78) Laboratory glassware. Principles of arrangement and design of measuring glassware

3. BASIC UNITS OF MEASUREMENT

3.1. Capacity unit

The unit of capacity is taken to be a cubic centimeter (cm); the name milliliter (ml) can be used.

Note. The term milliliter (ml) is usually used as a special name for cubic centimeter (cm) according to the International System of Units (SI).

3.2. Standard temperature

Standard temperature, i.e. the temperature at which the nominal volume (nominal capacity) is drained is 20 °C.

Note. When using pipettes in tropical conditions, it is permissible to take the standard water temperature as 27 °C rather than 20 °C, as recommended by GOST 29044.

4. ACCURACY OF VOLUME MEASUREMENT

Two accuracy classes are established:

1st - for higher measurement accuracy;

2nd - for less precise measurements.

5. RANGE OF CAPACITIES

Pipettes with one mark must be manufactured with a nominal capacity of: 0.5; 1; 2; 5; 10; 20; 25; 50; 100 and 200 ml.

For the needs of the national economy, it is allowed to manufacture pipettes with one mark with a nominal capacity of 10.77 ml.

Pipettes with a nominal capacity of 0.5 ml are manufactured without a reservoir, 1 ml - with and without a reservoir, 2 ml - with a reservoir for the 1st class and with and without a reservoir for the 2nd class.

All other sizes are for pipettes with reservoirs.

If necessary, pipettes of all sizes can be manufactured with safety reservoirs above the graduation mark.

Note. If necessary, pipettes of other nominal capacities may be manufactured provided that they comply with the essential requirements of this standard.

6. DETERMINATION OF CAPACITY

The capacity of a single-mark pipette is defined as the volume of water, expressed in milliliters, at 20°C, as described below.

Note. If the recommended temperature is 27 °C, then this temperature value should be set instead of 20 °C.

The pipette, in a vertical position, should be filled with liquid a few millimeters above the graduation mark, and the drop of the meniscus should then be adjusted to the mark by one of the methods described below:

a) the meniscus is installed so that the plane of the upper edge of the graduation mark is horizontal tangent to the lower point of the meniscus, the observer’s gaze is in the same plane;

b) the meniscus is installed so that the plane of the center of the mark is horizontal tangent to the lower point of the meniscus. The observer's gaze is directed upward towards this plane, while the front and rear parts of the mark should be simultaneously visible, simultaneously converging at the bottom of the meniscus.

Then remove any drops that adhered to the spout tip when the spout tip came into contact with the surface of the glass container.

After this, the liquid must be drained into another, slightly tilted glass vessel so that the drain tip is in contact with the inner wall of the vessel, but without moving them relative to each other during the drain and waiting period.

The pipette is released until the meniscus drops to the drain hole. To ensure that the drainage has finished, wait 3 s before the pipette is removed from the vessel. This applies to pipettes for which there is no set waiting time.

Note. A waiting period (approximately 3 seconds) is set to determine when to drain the liquid. There is no need to strictly adhere to this time. Be sure that the meniscus has reached the outlet end of the pipette before removing the pipette from the receiving vessel.

If a pipette has a waiting time of 15 s, this time must be maintained before removing the pipette from the receiving vessel.

Note. 1st class pipettes must be:

a) without a specific waiting time, when this time is approximately 3 s;

b) with a waiting time of 15 s.

Class 2 pipettes must have no specified waiting time as defined in subparagraph a.

7. VOLUME PERMISSIBLE ERROR LIMITS

7.1. The limits of permissible error of the nominal capacity should not exceed the values specified in Table 1.

Table 1


Nominal capacity	Permissible error limit
	1st class	2nd class

8. CONSTRUCTION

8.1. Material

Single mark pipettes should be made of glass with adequate chemical and thermal resistance and, if possible, free from visible defects and internal stress.

8.2. Form

Pipettes with a nominal capacity of 0.5 ml consist of a straight tube with a drain hole at the lower end.

Pipettes with a nominal capacity of 1 ml and class 2 pipettes with a nominal capacity of 2 ml may have the design shown in Figure 1 or may have a reservoir as indicated for larger sizes.

Damn.1. Pipette shape

All large pipettes consist of a reservoir with a suction and drain tube. These parts must be straight and aligned.

The shape of the pipettes must correspond to that shown in Figure 1.

If a safety reservoir is provided, it is approximately spherical in shape and is located between the graduation mark and the top of the pipette.

For the needs of the national economy, it is allowed to manufacture pipettes with one mark in the following designs:

1 - straight (Fig. 2);

1a - straight lines with a spare tank (Fig. 2);

2 - with extension (Fig. 2);

2a - with expansion and spare tank (Fig. 2).

Damn.2. Pipette 1-2-50

An example of a symbol for a pipette used for the needs of the national economy, class 1, class 2, nominal capacity 50 ml:

Pipette 1-2-50 GOST 29169-91

8.3. Dimensions

The dimensions of the pipettes must correspond to those indicated in Tables 2 and 3.

table 2

Dimensions in mm


Size name	Nominal capacity, ml

Total length, no more:
straight pipettes
pipettes with reservoir
Length of suction tube, not less
Length of drain pipe, not less
Inner diameter at the graduation mark, no more
Outer diameter of drain pipe (maximum deviation ±1 mm)
Tank diameter, no more
Straight pipette tube diameter, no more

Only for pipettes with reservoir.

For straight pipettes - maximum internal diameter; for pipettes with a reservoir - the maximum internal diameter of the suction tube.

The calculated minimum distance from the graduation mark to the end of the drain hole is 120 mm (see Table 3).

For pipettes used for the needs of the national economy.

Table 3


Size name	Meaning
Distance of the calibration mark from the top of the pipette, not less
Distance from the calibration mark to the top of the tank, not less
Distance from the calibration mark to the drain cone of straight pipettes, not less
Pipette wall thickness, not less:
with a reservoir with a nominal capacity of 1 and 2 ml
other pipettes
Safety tank diameter
Distance from the top of the pipette to the bottom of the safety tank, no more
Distance from the top of the pipette to the top of the safety reservoir, not less

Note. Allowed for pipettes used for the needs of the national economy:

1) the thickness of the expansion wall for pipettes with a reservoir is at least 0.4 mm;

2) the value of the diameter of the safety tank - take it as a size for reference.

The outer diameter of the drain tube and the diameter of the straight pipette tube for pipettes used for national household needs are recommended.

8.4. The top of the pipette should be perpendicular to the axis of the pipette and free from any defects that could interfere with the establishment of the meniscus.

The ends of the glass tubes should be fused or polished smooth with a slight bevel outward.

8.5. Storage tank

The main body of the reservoir is cylindrical, with the exception of pipettes with a nominal capacity of 1 and 2 ml, which may have an oval reservoir if required. The two ends of the tank should gradually transition into the suction and drain pipes. To facilitate drainage of the pipette, the connections should be cone-shaped.

8.6. Drain tip

8.6.1. The drain tip should be strong, smooth, tapered without sharp narrowings at the drain hole.

8.6.2. The end of the drain tip should be treated using one of the methods described below:

a) smoothly ground perpendicular to the axis, slightly beveled outward and melted;

b) smoothly ground perpendicular to the axis and slightly beveled outward;

c) for pipettes used for the needs of the national economy, cut off and melted.

Fire polishing reduces the risk of fracture during use, but should not cause contraction, as specified in paragraph 8.6.1, or internal stress.

8.7. Drain time

The drain time is defined as the time required for the water meniscus to descend from the graduation mark to the drain hole.

The drain time is determined with the pipette in a vertical position and the receiving vessel should be slightly tilted so that the tip of the drain tube is in contact with the inner wall of the vessel, but without moving relative to each other.

Thus, the drain time should be within the limits specified in Table 4.

Table 4


Accuracy class	Drain time, s, for pipettes with nominal capacity, ml

1st class without specific waiting time:


1st class with waiting time 15 s:
no less
no more
2nd class:


Maximum permissible difference between actual and indicated drain time

If the draining time is indicated on the pipette in accordance with clause 10.2, then the actual and indicated time values should not differ by more than the value indicated in Table 4.

For pipettes used for the needs of the national economy.

9. GRADING MARK

10. LETTERINGS

10.1. All pipettes must be marked with the following markings:

a) a number indicating the nominal capacity and the abbreviation cm or ml to indicate the units in which the pipette is calibrated (see note to clause 3.1);

c) the letter "O", indicating that the pipette is intended to discharge the corresponding nominal capacity;

d) designation 1 or 2 to indicate the accuracy class of graduated pipettes;

e) trademark or name of the manufacturer;

f) the waiting time, if set, in the form "0+15 s".

a) designation of this standard;

b) drain time in seconds.

10.3. The permissible error limit for the nominal capacity in accordance with Table 1 can be applied to all pipettes, for example ±. . . ml.

11. GRADUATION MARKS, NUMBERS AND INSTRUCTIONS

11.1. All numbers and symbols must be of such size and shape as to be clearly visible under normal conditions of use.

11.2. The graduation mark, numbers and symbols must be clearly visible and indelible.

12. COLOR CODING

If color coding is used, it must be performed in accordance with the requirements of regulatory and technical documentation approved in the prescribed manner.

APPENDIX A. RELATIONSHIP BETWEEN CAPACITY AND PERMISSIBLE ERROR LIMIT FOR CLASS 1 PIPETTES

APPENDIX A

RELATIONSHIP BETWEEN CAPACITY AND PERMISSIBLE ERROR LIMIT FOR CLASS 1 PIPETTES IN ACCORDANCE WITH THE REQUIREMENTS OF clause 4.4 GOST 29044

Damn.3

APPENDIX B. RELATIONSHIP BETWEEN PERMISSIBLE ERROR LIMITS AND MENISCU DIAMETER FOR CLASS 1 PIPTETES

APPENDIX B

RELATIONSHIP BETWEEN THE LIMITS OF PERMISSIBLE ERROR AND THE DIAMETER OF THE MENISCUS FOR PIPETTES 1ST CLASS IN ACCORDANCE WITH THE REQUIREMENTS OF clause 4.7 GOST 29044

APPENDIX 1. ADDITIONAL REQUIREMENTS REFLECTING THE NEEDS OF THE NATIONAL ECONOMY

ANNEX 1

13. Test methods

13.1. The nominal capacity of pipettes should be checked in accordance with the regulatory and technical documentation approved in the prescribed manner.

13.2. The dimensions of the pipettes (clause 8.3) should be checked with a universal measuring instrument in accordance with GOST 166 and GOST 427.

13.3. The draining time of pipettes should be checked according to clause 8.7 of this standard.

14. Packaging, transportation and storage

14.1. Pipettes are packaged in such a way as to ensure their safety during transportation and storage.

14.2. Pipettes packaged according to clause 14.1 are placed in transport containers, where they must be protected from movement and damage.

14.3. Pipettes should be transported in clean covered vehicles.

Electronic document text
prepared by Kodeks JSC and verified against:
official publication
Laboratory glassware and equipment.
Pipettes. Burettes: Sat. GOST. -
M.: Standartinform, 2008